Laboratory Optimizer for Mass Testing is an open-source software solution designed to enhance the efficiency and capacity of laboratories conducting mass screenings for infectious diseases like COVID-19. By implementing advanced pool testing algorithms and data analytics, LOMT enables laboratories to test significantly more individuals using existing resources, thereby reducing both costs and turnaround times. The software seamlessly integrates with current laboratory workflows, offering a user-friendly interface accessible via web browsers on various devices, including desktops, laptops, tablets, and smartphones. LOMT supports both automated and manual laboratory settings, providing flexibility in its application. Key Features and Functionality: - End-to-End Automation: LOMT automates the entire pool testing process, from determining optimal pooling strategies based on prevalence and operational constraints to designing pool mixing protocols, tracking specimens and assays, and decoding assay results. - Advanced Pooling Algorithms: The software supports both classic and advanced pooling algorithms, including combinatorial pooling for near-complete results in a single round and binary splitting for optimal assay savings. - Multi-Condition Segmentation: LOMT analyzes sample data to segment specimens based on different prevalence rates and testing conditions, selecting the most efficient pooling strategy for each segment or reverting to individual testing when pooling is not advantageous. - Flexible Integration: LOMT can be integrated with Laboratory Information Management Systems and laboratory automation equipment, or used as a standalone application in manual laboratories, including mobile labs and infirmaries. Primary Value and Problem Solved: LOMT addresses the critical need for scalable and cost-effective mass testing during infectious disease outbreaks. By optimizing pool testing workflows, it allows laboratories to: - Increase Testing Capacity: Test up to 30 times more individuals using existing resources. - Reduce Costs: Lower testing expenses by up to 15 times through efficient use of assays and reagents. - Enhance Efficiency: Streamline laboratory operations, reducing the time required for testing and improving overall throughput. By implementing LOMT, laboratories can effectively manage large-scale testing demands, ensuring timely and accurate results while conserving resources.