Dianthus is a plate-based, microfluidics-free affinity screening platform designed to tackle the most challenging drug discovery campaigns. Utilizing breakthrough Spectral Shift and Temperature Related Intensity Change (TRIC technologies, Dianthus measures molecular interactions in solution, providing high-quality, mass-independent data. This makes it particularly effective for applications involving PROTAC binary and ternary complexes, fragment libraries, and intrinsically disordered proteins. By delivering results with high signal-to-noise ratios and requiring minimal assay development, Dianthus ensures actionable insights from real-life samples, even in the presence of aggregates or impurities. Its compatibility with automation solutions and low sample consumption further streamline the screening process, enabling researchers to confidently identify and validate hits, ultimately accelerating the drug discovery pipeline. Key Features and Functionality: - Dual Measurement Technologies: Incorporates Spectral Shift and TRIC methods to detect molecular interactions with high sensitivity. - In-Solution, Mass-Independent Measurements: Performs analyses in solution without the need for immobilization, preserving native conditions and ensuring accurate results. - Broad Affinity Range Detection: Capable of measuring binding affinities from picomolar to millimolar, capturing both strong and weak binders. - Minimal Sample Consumption: Requires small amounts of target and compounds, conserving valuable resources. - Automation Compatibility: Designed for seamless integration into automated workflows, supporting uninterrupted, high-throughput screening. - Low Maintenance: Microfluidics-free design eliminates regular maintenance and downtime, ensuring continuous operation. Primary Value and Problem Solved: Dianthus addresses the limitations of traditional affinity screening methods, such as surface plasmon resonance (SPR, which often struggle with complex targets and require extensive assay development. By providing high-quality, in-solution data with minimal sample requirements and compatibility with real-life samples, Dianthus empowers researchers to confidently advance their drug discovery projects, even when faced with challenging targets and conditions. Its automation-friendly design and low maintenance needs further enhance efficiency, reducing time and costs associated with hit identification and validation.